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Differential Expression of Caveolin-1 in Lipopolysaccharide-Activated Murine Macrophages

机译：Caveolin-1在脂多糖激活的小鼠巨噬细胞中的差异表达。

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Five reciprocal cycles of subtractive hybridization using cDNA generated from fibroblasts with normal lipopolysaccharide (LPS) responsiveness (lpsn) and from hyporesponsive (lpsd) fibroblasts have led to the finding that caveolin-1 is expressed at markedly higher levels of mRNA in lpsd than in lpsn fibroblasts. Caveolin-1 message can also be readily detected via reverse transcription-PCR in the RAW264.7 and J774.1 macrophage-like cell lines as well as in primary thioglycolate (TG)-elicited mouse peritoneal macrophages. In RAW264.7 cells, both caveolin-1 mRNA and protein levels are down-regulated by LPS. In TG-elicited C3HeB/FeJ peritoneal macrophages, in contrast, expression of both caveolin-1 protein and mRNA is up-regulated in vitro in response to LPS stimulation. The up-regulation of caveolin-1 protein expression in C3HeB/FeJ peritoneal macrophages can be demonstrated at concentrations as low as 1.0 pg of LPS/ml. However, LPS concentrations approximately 4 orders of magnitude higher (104 pg/ml) were required to stimulate the LPS-hyporesponsive C3H/HeJ mice peritoneal macrophages such that significant caveolin-1 protein up-regulation was detected. Caveolin-1, a principal component of plasmalemmal caveolae, has been reported as a potentially important regulator for signal transduction during cellular stimulation. The results described in this report suggest that caveolin-1 expression may be associated with LPS signaling/internalization.
机译：使用具有正常脂多糖（LPS）反应性（lpsn）的成纤维细胞和反应低下（lpsd）的成纤维细胞生成的cDNA，通过消减杂交的五个往复循环导致发现Caveolin-1在lpsd中的表达水平明显高于lpsn中的mRNA表达。成纤维细胞。也可以通过逆转录-PCR在RAW264.7和J774.1巨噬细胞样细胞系中以及在硫代乙醇酸酯（TG）诱导的小鼠腹膜巨噬细胞中轻易检测到Caveolin-1信息。在RAW264.7细胞中，LPS下调了Caveolin-1 mRNA和蛋白水平。相反，在TG引起的C3HeB / FeJ腹膜巨噬细胞中，响应LPS刺激，caveolin-1蛋白和mRNA的表达在体外均被上调。 C3HeB / FeJ腹膜巨噬细胞中Caveolin-1蛋白表达的上调可以在低至1.0 pg LPS / ml的浓度下得到证实。但是，刺激LPS低反应性C3H / HeJ小鼠腹膜巨噬细胞需要大约4个数量级（104 pg / ml）的LPS浓度，从而检测到明显的Caveolin-1蛋白上调。 Caveolin-1是质膜小窝的主要成分，据报道是细胞刺激过程中信号转导的潜在重要调节剂。该报告中描述的结果表明，caveolin-1表达可能与LPS信号传导/内在化有关。

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